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Predicted subcellular localization distribution of the proteome from three reference strains of Renibacterium salmoninarum . (A) Strain ATCC 33209, comprising 2,926 predicted proteins. (B) Strain DJ2R, comprising 2,925 predicted proteins. (C) Strain H2, comprising 2,925 predicted proteins. (D) Summary of the sequential filtering steps applied to the three proteomes, yielding 15 candidate proteins for epitope prediction. In all strains, the majority of proteins were classified as cytoplasmic and membrane-associated, while a smaller proportion corresponded to extracellular, cell surface-associated, and proteins with unknown subcellular localization.
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The heatmap shows the number of days vertically and the C. albicans isolates injected each in 6 mice horizontally. Each cell is color coded according to the number of moribund mice identified on a daily basis and which is represented by the color scale. FL1-FL6 are the fluconazole-resistant isolates while CS1-CS6 are the caspofungin-resistant isolates. The control group was infected by the reference strain C. albicans SC5314.

Journal: PLOS One

Article Title: Diverse genetic pathways drive convergent fluconazole and caspofungin resistance phenotypes in Candida albicans

doi: 10.1371/journal.pone.0360234

Figure Lengend Snippet: The heatmap shows the number of days vertically and the C. albicans isolates injected each in 6 mice horizontally. Each cell is color coded according to the number of moribund mice identified on a daily basis and which is represented by the color scale. FL1-FL6 are the fluconazole-resistant isolates while CS1-CS6 are the caspofungin-resistant isolates. The control group was infected by the reference strain C. albicans SC5314.

Article Snippet: The reference strain C. albicans SC5314 (ATCC MYA-2876) was used as control.

Techniques: Injection, Control, Infection

Ergosterol content is expressed as a percentage of total sterols normalized to biomass weight. The reference strain C. albicans SC5314 was used as control and its quantity of ergosterol is represented by the striped bar. The p-values for the fluconazole-resistant and caspofungin-resistant groups of isolates relative to the control are shown.

Journal: PLOS One

Article Title: Diverse genetic pathways drive convergent fluconazole and caspofungin resistance phenotypes in Candida albicans

doi: 10.1371/journal.pone.0360234

Figure Lengend Snippet: Ergosterol content is expressed as a percentage of total sterols normalized to biomass weight. The reference strain C. albicans SC5314 was used as control and its quantity of ergosterol is represented by the striped bar. The p-values for the fluconazole-resistant and caspofungin-resistant groups of isolates relative to the control are shown.

Article Snippet: The reference strain C. albicans SC5314 (ATCC MYA-2876) was used as control.

Techniques: Control

Chitin concentration in mg/mL of 100 mg biomass wet weight pellet were used. The reference strain C. albicans SC5314 was used as control and its chitin concentration is represented by the striped bar. The p-values for the fluconazole-resistant and caspofungin-resistant groups of isolates relative to the control are shown.

Journal: PLOS One

Article Title: Diverse genetic pathways drive convergent fluconazole and caspofungin resistance phenotypes in Candida albicans

doi: 10.1371/journal.pone.0360234

Figure Lengend Snippet: Chitin concentration in mg/mL of 100 mg biomass wet weight pellet were used. The reference strain C. albicans SC5314 was used as control and its chitin concentration is represented by the striped bar. The p-values for the fluconazole-resistant and caspofungin-resistant groups of isolates relative to the control are shown.

Article Snippet: The reference strain C. albicans SC5314 (ATCC MYA-2876) was used as control.

Techniques: Concentration Assay, Control

The reference strain C. albicans SC5314 was used as control and R6G efflux in function of time is represented by the red curve. The changes in R6G efflux were quantified by measuring OD at 527 nm over time in each fluconazole-resistant isolate (FL1 to FL6; black curves differentiated by distinct symbols) compared to the control and the differences were statistically significant. Each data point represents the mean value of three replicates. The standard deviation for each data point is shown in the table. The p-values for each isolate relative to the control are shown.

Journal: PLOS One

Article Title: Diverse genetic pathways drive convergent fluconazole and caspofungin resistance phenotypes in Candida albicans

doi: 10.1371/journal.pone.0360234

Figure Lengend Snippet: The reference strain C. albicans SC5314 was used as control and R6G efflux in function of time is represented by the red curve. The changes in R6G efflux were quantified by measuring OD at 527 nm over time in each fluconazole-resistant isolate (FL1 to FL6; black curves differentiated by distinct symbols) compared to the control and the differences were statistically significant. Each data point represents the mean value of three replicates. The standard deviation for each data point is shown in the table. The p-values for each isolate relative to the control are shown.

Article Snippet: The reference strain C. albicans SC5314 (ATCC MYA-2876) was used as control.

Techniques: Control, Standard Deviation

The reference strain C. albicans SC5314 was used as control and biofilm content is represented by the striped bar. Biofilm biomass production was quantified by measuring OD at 590 nm upon crystal violet release. The p-values for the groups for the fluconazole-resistant and caspofungin-resistant groups of isolates relative to the control are shown.

Journal: PLOS One

Article Title: Diverse genetic pathways drive convergent fluconazole and caspofungin resistance phenotypes in Candida albicans

doi: 10.1371/journal.pone.0360234

Figure Lengend Snippet: The reference strain C. albicans SC5314 was used as control and biofilm content is represented by the striped bar. Biofilm biomass production was quantified by measuring OD at 590 nm upon crystal violet release. The p-values for the groups for the fluconazole-resistant and caspofungin-resistant groups of isolates relative to the control are shown.

Article Snippet: The reference strain C. albicans SC5314 (ATCC MYA-2876) was used as control.

Techniques: Control

Distribution of the number of screened resistance genes per E. coli -like enterobacterial isolate from anaerobic digester influent and biofertilizer

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Distribution of the number of screened resistance genes per E. coli -like enterobacterial isolate from anaerobic digester influent and biofertilizer

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques:

Absolute frequency of selected resistance-gene detection in E. coli -like enterobacterial isolates from anaerobic digester influent and biofertilizer

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Absolute frequency of selected resistance-gene detection in E. coli -like enterobacterial isolates from anaerobic digester influent and biofertilizer

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques:

Antimicrobial susceptibility profiles of E. coli -like enterobacterial isolates from anaerobic digester influent and biofertilizer. Legend: GEN (Gentamicin), AMC (Amoxicillin-clavulanate), CLO (Chloramphenicol), CAZ (Ceftazidime), MER (Meropenem), LVX (Levofloxacin), TET (Tetracycline). Colors represent CLSI susceptible, intermediate, and resistant classifications

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Antimicrobial susceptibility profiles of E. coli -like enterobacterial isolates from anaerobic digester influent and biofertilizer. Legend: GEN (Gentamicin), AMC (Amoxicillin-clavulanate), CLO (Chloramphenicol), CAZ (Ceftazidime), MER (Meropenem), LVX (Levofloxacin), TET (Tetracycline). Colors represent CLSI susceptible, intermediate, and resistant classifications

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques:

Distribution of the number of tested antimicrobial agents classified as resistant (R) per presumptive E. coli -like enterobacterial isolate from effluent and biofertilizer

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Distribution of the number of tested antimicrobial agents classified as resistant (R) per presumptive E. coli -like enterobacterial isolate from effluent and biofertilizer

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques:

Biocide inhibition-zone profiles of E. coli -like enterobacterial isolates from effluent and biofertilizer. Data are presented as mean ± SD. The dashed line indicates the 6-mm disk diameter. H 2 O 2 inhibition zones differed significantly between matrices after Benjamini–Hochberg correction (q = 0.00493). LA 2% (2% Lactic Acid); BAC 0.28% (0.28% Benzalkonium chloride), H 2 O 2 3% (3% hydrogen peroxide); CHX 1% (1% chlorhexidine digluconate)

Journal: World Journal of Microbiology & Biotechnology

Article Title: Phenotypic and genotypic assessment of antimicrobial and biocide resistance in enterobacteria from anaerobic digestion of dairy waste: implications for environmental and public health

doi: 10.1007/s11274-026-05319-9

Figure Lengend Snippet: Biocide inhibition-zone profiles of E. coli -like enterobacterial isolates from effluent and biofertilizer. Data are presented as mean ± SD. The dashed line indicates the 6-mm disk diameter. H 2 O 2 inhibition zones differed significantly between matrices after Benjamini–Hochberg correction (q = 0.00493). LA 2% (2% Lactic Acid); BAC 0.28% (0.28% Benzalkonium chloride), H 2 O 2 3% (3% hydrogen peroxide); CHX 1% (1% chlorhexidine digluconate)

Article Snippet: All isolates and the E. coli ATCC 25,922 reference strain yielded 6-mm measurements with lactic acid, indicating that the tested LA condition was non-discriminatory.

Techniques: Inhibition

Predicted subcellular localization distribution of the proteome from three reference strains of Renibacterium salmoninarum . (A) Strain ATCC 33209, comprising 2,926 predicted proteins. (B) Strain DJ2R, comprising 2,925 predicted proteins. (C) Strain H2, comprising 2,925 predicted proteins. (D) Summary of the sequential filtering steps applied to the three proteomes, yielding 15 candidate proteins for epitope prediction. In all strains, the majority of proteins were classified as cytoplasmic and membrane-associated, while a smaller proportion corresponded to extracellular, cell surface-associated, and proteins with unknown subcellular localization.

Journal: Frontiers in Bioinformatics

Article Title: Reverse vaccinology and immunoinformatics-driven design of a multiepitope vaccine candidate against Renibacterium salmoninarum

doi: 10.3389/fbinf.2026.1899416

Figure Lengend Snippet: Predicted subcellular localization distribution of the proteome from three reference strains of Renibacterium salmoninarum . (A) Strain ATCC 33209, comprising 2,926 predicted proteins. (B) Strain DJ2R, comprising 2,925 predicted proteins. (C) Strain H2, comprising 2,925 predicted proteins. (D) Summary of the sequential filtering steps applied to the three proteomes, yielding 15 candidate proteins for epitope prediction. In all strains, the majority of proteins were classified as cytoplasmic and membrane-associated, while a smaller proportion corresponded to extracellular, cell surface-associated, and proteins with unknown subcellular localization.

Article Snippet: Candidate protein selection was performed independently and in parallel using the proteomes of 3 R. salmoninarum strains: the reference strain ATCC 33209 (2,926 proteins; ) and the field strains DJ2R (2,925 proteins; ) and H2 (2,925 proteins; ).

Techniques: Membrane